摘要:
研究了X射线和重离子照射对人微血管内皮细胞的增殖、迁移、管样结构形成及基质金属蛋白酶表达的影响.Transwell迁移实验结果显示,照射24 h后,非毒性照射剂量照射能抑制HMEC-1细胞的迁移,且重离子照射抑制内皮细胞迁移能力较X射线的强.基质胶实验显示,重离子照射可显著抑制自发性管样结构的形成,但X射线照射的抑制作用不明显.同时,明胶酶谱法证实了重离子照射可明显抑制HMEC-1细胞中基质金属蛋白酶( MMP-2和MMP-9)的表达,而X射线照射对此类酶几乎无任何影响,显示了重离子较X射线的肿瘤放疗优势所在.
关键词:
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重离子
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X射线
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辐照
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血管生成
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肿瘤
Abstract:
The study aims to explore the effect of 12C6+ion and X-ray on proliferation, migration, tube formation and MMPS of human microvascular endothelial cells ( HMEC-1 ). Transwell migration experiment showed that the radiation could inhibit the migration of HMEC-1 in sub-lethal dose 24 h after irradiation. Furthermore, the inhibition ability of 12C6+ion was stronger than that of X-ray. Matrigel experiment indicated that 12C6+ion suppressed the tube formation of HMEC-1 spontaneously. However, the inhibitory effect of X-ray had no significance. Meanwhile, Gelatin Zymography showed the expression of MMP-2 was inhibited obviously by 12C6+ion, while X-ray had little effect on the expression of MMP-2. In conclusion, 12C6+ion is superior to X-ray in radiotherapy of tumor.
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