Keywords: 
• 超高效液相色谱-串联质谱(UPLC-MS/MS) /
• 二硝基苯胺 /
• 除草剂 /
• 蔬菜 /
• 水果 /
• 粮谷

Abstract: A method of ultra performance liquid chromatography-tandem mass spectrom-etry(UPLC-MS/MS)was developed for determination of 8 kinds of dinitroaniline herbi-cides(trifluralin,pendimethalin,butralin,isopropalin,dinitramine,nitralin,oryzalin, prodiamine)in spinach,apple and soybean.Afer homogenization,5.0 g sample was soaked with 7 mL water for 1 h.Then 3 g NaCl was added,and the sample was extrac-ted twice by 25 mL hexane saturated acetonitrile solution.After concentration,the extract was put through C18 column and eluted by 5 mL acetonitrile.The eluated was then concentrated and dissolved with 2.0 mL hexane-acetone mixture(2:8,V/V).The preparation was cleaned by Envi-Carb column and eluted with 5.0 mL hexane-acetone. The residue was dissolved by 50% acetonitrile aqueous solution(contain 0.1% formic acid),and determined by UPLC-ESI/MS/MS.Under the optimized chromatographic conditions,8 target compounds were obtained.Confirmation was achieved using electro-spray ionization(ESI)in positive mode with multiple reaction monitoring(MRM),and external standard method was used for quantification.The results show that 8 dinitroa-niline herbicides have good linearity,the linearity is more than 0.99.T he limits of quantification(LOQs)are 10 μg/kg.The mean recoveries are between 70.0% and 120%at the spiked levels of 10-200 μg/kg.The RSD of the method are between 0.56% and 23.0%.T he method is precise,sensitive and accurate,which is suitable for confirma-tion and quantification of dinitroaniline herbicides in vegetables,fruits and grains.