摘要:
采用超高效液相色谱-串联Orbitrap质谱(UHPLC-Q/Exactive)技术分析湿热质(n=8)和平和质(n=13)志愿者尿液,运用质谱数据建立无监督的火山模型和有监督的正交偏最小二乘判别分析模型(OPLS-DA)筛选湿热质的特征代谢物,并分析湿热质与平和质志愿者间代谢表型的差异,通过特征代谢物的代谢通路分析,研究湿热质形成的分子作用机理.结果表明,湿热质和平和质的代谢表型存在显著差异,通过火山模型可筛选出22个差异显著的代谢物,通过OPLS-DA模型可筛选出29个特征代谢物.代谢通路分析结果表明,湿热质志愿者精氨酸和脯氨酸代谢,组氨酸代谢,甘氨酸、丝氨酸和苏氨酸代谢等通路发生了紊乱,这为针对湿热质人群实施精准营养提供了新的思路.
Abstract:
To reveal the underlining molecular mechanism for the formation of damp and hot constitution,and also promote personalized nutrition for people with that constitution,a dozens of volunteers were recruited to conduct a metabolic research.The volunteers were diagnosed by Chinese physician,and people who were mild constitution as well as damp and hot constitution were screened.Afterwards,the first morning urine was collected and analyzed using ultra high performance liquid chromatography coupled with high revolution orbitrap mass spectrometry (UHPLC-Q/Exactive).Mass spectra were then digitalized into high demission data matrix.Meanwhile,a volcano model and an orthogonal partial least squares discriminant analysis (OPLS-DA) were performed to screen the most relevant metabolites which could discriminate damp and hot constitution.Furthermore,the pathway analysis was conducted to reveal the molecular mechanism for the formation of damp and hot constitution.Result show that there was a significant difference between damp and mild constitution,and 22 metabolites were proven to be significantly different by volcano model.However,29 metabolites screened by OPLS-DA model were the most relevant with damp and hot constitution.Pathway analysis base on the screened metabolites showed that dysregulation in arginine and proline metabolism,histidine metabolism,glycine,serine and threonine metabolism might account for the formation of damp and hot constitution.
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