摘要:
文章介绍了作者所在的实验小组近年来在用表面微纳米技术研究细胞生物学方面取得的进展.由于细胞的尺寸在数微米到数十微米之间,应用微纳米技术可以精细地调控细胞微观环境.作者所在的实验小组应用微流控系统以及表面化学修饰等方法,对细胞正常行为和病理行为进行了一系列研究.通过设计力学刺激装置,对细胞骨架的主要成分———肌动蛋白对细胞的形状以及粘附、迁移等行为的影响进行了系统研究,实现了对肌动蛋白拉伸过程的全程实时动态观察,发现一次拉伸刺激就可以导致肌动蛋白细胞骨架变短,并可以促进肌动蛋白纤维的重组过程.此外,作者所在的实验小组还构建了表面三维结构,以模拟生物体内的拓扑结构,并结合微流控、细胞图案化和多种细胞共培养技术,构建了体外细胞培养和相互作用研究模型,发现细胞形状可以影响其极性和迁移的方向,以及神经细胞突起可以感受表面层粘连蛋白的浓度梯度等一系列有趣的现象,并对其机理进行了探讨.
Abstract:
We report the recent progress in our studies of cell biology using micro-/nano-technology.Cells have a size of several to tens of microns,which makes them easily manipulated by micro-/nano-technology.The shape of the cell influences the alignment of the actin cytoskeleton,which bears the main forces of the cell,maintains the shape,and mediates a series of biochemical reactions.We invented a stretching device and studied the real-time actin filament dynamics under stretch.We found that one stretch cycle shortened the actin filaments and promoted their reassembly process.Cell migration is a complex mechanical process.We found that cell geometry determines the cell polarity and migration direction.We fabricated three-dimensional surfaces to mimic the topography in vivo,and further built a cell culture model by integrating the three-dimensional surface,microfluidics,cell patterning,and coculturing of multiple cell types.We also investigated the neuronal guidance by surface patterning.
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