摘要:
选用12C6+离子束对阿维链霉菌诱变选育高产菌株与原始菌株进行辐照诱变,研究其累进辐照效应。实验结果表明,在辐照剂量为10Gy时,原始菌株比诱变高产菌株存活率高,抗辐射能力强;辐照剂量高于30Gy时,诱变高产菌株比原始菌株存活率高,抗辐射能力强。原始菌株正突变率最高的辐照剂量为50Gy,致死率99.43%,正突变率最高,达34.2%;对诱变高产菌株辐照剂量为30Gy,致死率94.97%,正突变率最高,达23.5%。累进辐照效应降低了最佳辐照剂量。
关键词:
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辐照诱变
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累进辐照
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效应
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存活率
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正突变率
Abstract:
Mutagenic effect on the mutant high-producing strain ZJAV-Y1-203 and the original strain ZJAV-A1 irradiated by ion beam of 12 C6 + have been investigated.The experimental results indicated that the original strain has higher survival rate and stronger resistance to radiation than mutant high-producing Strain at dose of 10 Gy.The mutant high-producing strain has higher survival rate and stronger resistance to radiation than the original strain at the dose higher than 30 Gy.The lethality was 97% and the highest rate of orthomutation was 34.2%,when ZJAV-A1 was irradiated by 50 Gy 12 C6 + beam.The lethality was 94.97% and the highest rate of orthomutation was 23.5% when ZJAV-Y1-203 was irradiated by 30 Gy 12 C6 +beam.The best radiation dose is decreased by progressivity irradiation.