摘要:
利用UPLC-LTQ-Orbitrap-MS技术结合文献报道的鬼箭羽已知化学成分及其裂解规律快速鉴定鬼箭羽药材中的化学成分.利用文献中鬼箭羽化学成分的分子式、相对分子质量、碎片离子等信息建立鬼箭羽快速识别数据库, 依据化合物的精确分子质量、色谱保留时间、特征碎片离子等信息对已知成分进行验证;对于鬼箭羽中未知成分, 通过总结同类化合物的质谱裂解规律, 并查阅相关文献确定其相对分子质量、特征碎片离子、色谱保留时间等信息, 并进行结构表征.实验共鉴定得到65种成分, 包括36种黄酮类、4种酚酸类、7种三萜类、12种脂肪族和6种其他类成分, 其中, 有28种化合物是首次在鬼箭羽中发现.该方法可以快速、全面地分析鬼箭羽药材中的化学成分, 可为鬼箭羽药材的质量控制研究奠定基础.
Abstract:
Liquid chromatography-mass spectrometry has many advantages, such as high separating efficiency, good sensitivity and strong specifity, which is widely used for rapid separation and identification of chemical components in traditional Chinese medicines.Euonymus alatus is a Chinese medicinal herb, which has been used for thousands of years in China and the medicinal part is the branch wings of Euonymus.It is often used to treat diabetes, and it is commonly used as a traditional Chinese medicine for the treatment of malaria, gynecological diseases in recent years.As a traditional Chinese medi-cine for the treatment of diabetes, the active ingredients of Euonymus alatus are not clear.The chemical composition of Euonymus alatus can be obtained by extraction and separation, but this method has some limitations, such as trace chemical components are not easy to obtain, the time required is longer, the consumption of raw materials is more, etc.A method for combining UPLC-LTQ-Orbitrap-MS with known chemical composition databases of Euonymus alatus and summarizing the mass spectrometry cleavage rules of various compounds was established to identify multiple chemical components in Euonymus alatus.Firstly, according to the literature, a database of known compounds of Euonymus alatus was established, including names of compounds, relative molecular masses, fragment ions, molecular formulas, etc.Then, the law of mass spectrometry cleavage of different types of compounds was summarized, including flavonoids, phenolic acids, triterpenes, aliphatics, etc.Finally, the accuracy of the relative molecular mass, fragment ions, retention behaviors were compared with the database and the law of cracking was analyzed, so as to the structure of the compound was determined.A total of 65 components were identified, including 36 flavonoids, 4 phenolic acids, 7 triterpenes, 12 aliphatics, and 6 other components.29 compounds were found for the first time in Euonymus alatus.This method is rapid and comprehensive for analysis of the chemical constituents of complicated herbal extracts.
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