摘要:
运用基于液相色谱-质谱联用技术的代谢组学方法研究了不同卷烟烟气暴露对大鼠内源小分子代谢物组的影响.分别建立了大鼠血清和尿液的代谢轮廓谱,分析了烟气暴露7天、14天和30天时对照组大鼠、普通卷烟暴露组大鼠及含有天然本草添加剂的某品牌卷烟暴露组大鼠的血浆和尿液样本,采用偏最小二乘判别分析(PLS-DA)对数据进行模式识别.结果表明,普通卷烟和天然本草添加卷烟均会影响大鼠整体代谢状态,干扰大鼠磷脂、能量代谢,并对其造成氧化损伤,但天然本草添加卷烟对大鼠的损伤程度低于普通卷烟.在烟气暴露30天时,一些重要标志物在各组相对含量的变化进一步证实了天然本草添加卷烟可降低烟气对大鼠整体代谢的影响,减轻烟气造成的损伤.因此,在烟草中加入天然本草添加剂可在一定程度上减少烟气对机体的伤害,改善体内因烟气干扰而紊乱的磷脂和能量代谢.
Abstract:
The influences of different cigarette smoke exposure on endogenous small molecular metabolome were evaluated by metabolomics research on the basis of liquid chromatography-mass spectrometry (LC/MS).Plasma and urine samples from normal control rats, ordinary cigarette smoked rats and a certain commercial cigarette with herbal additives smoked rats were analyzed respectively to obtain the metabolic profiling.The rats were exposed to cigarette smoke for one week, half a month and one month.The data were processed by the method of partial least squares discriminant analysis (PLS-DA).The potential biomarkers were screened out according to VIP (variable importance in the projection) and identified by database and standards.The PLS-DA results at different cigarette smoke exposing periods showed that the metabolism of rats was affected by both ordinary cigarette smoke exposed group and the certain commercial cigarette smoke exposed group, mainly including phospholipid metabolism, energy metabolism and oxidative damage.However, the damage of the commercial cigarette smoked rats was lower than that of ordinary cigarette smoked rats.The relative concentration changes of some important biomarkers among the three groups, especially in one month, further confirmed that the herbal additives in the cigarette may relieve the influence of cigarette smoke on the holistic metabolome of rats, and improve their interfered metabolism, such us phospholipid and energy metabolism.
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