Keywords: 
• 黄泥煨制 /
• 人参皂苷 /
• 液相色谱-质谱

Abstract: Panax ginseng C .A .Mey (ginseng) is a traditional Chinese medicine herb , w hich has been widely used for the treatment of enhancing intelligence and improving immunity in China and Aisa .As a new resource food in China ,ginseng has a variety of processing methods which are used for different symptoms .The major active compo‐nents of ginseng are ginsenosides ,w hich demonstrate the ability to target a number of tissues and produce an array of pharmacological responses .In this study ,active compo‐nents from mud‐covered processed‐ginseng were studied whether the chemical composi‐tion of ginseng is safety and reliable .Rapid resolution liquid chromatography coupled with quadrupole‐time‐of‐flight tandem mass spectrometry (RRLC‐Q‐TOF MS/MS) was used to compare the white ginseng ,vinegar processed ginseng and mud‐covered pro‐cessed ginseng for the composition of ginsenosides .Chromatographic conditions were as follow s :ZORBAX SB‐C18 column (2.1 mm × 100 mm × 3.5 μm ) ,column temperature of 35 ℃ ,mobile phase of water‐acetonitrile gradient elution ,flow rate of 0.3 mL/min , injection volume of 5 μL .Thirty‐one ginsenosides were identified by the comparison of the retention times of the standard compounds and the accurate mass obtained from RRLC‐Q‐TOF MS/MS .A few minor ginsenosides ,such as ginsenoside F2 and Rg3 , were detected by the comparison of white ginseng with processed‐ginseng .The method is applicable to the research of compounds in the processed Chinese herbs .