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电喷雾萃取电离质谱在线检测卷烟主流烟气
Online Analysis of Mainstream Cigarette Smoke by Extractive Electrospray Ionization Mass Spectrometry
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摘要: 卷烟主流烟气是组成复杂、动态变化的气溶胶。在烟气捕集和处理过程中，具有活性的烟气成分会发生变化，从而使常规的离线分析方法无法准确地反映烟气的化学组成。本研究建立了一种用于卷烟主流烟气在线检测的电喷雾萃取电离质谱(EESI-MS)方法。通过单通道吸烟机对卷烟进行抽吸，卷烟主流烟气无需预处理而被直接引入 EESI-MS 仪中，得到其质谱图。在优化的工作条件下，通过碰撞诱导解离(CID)实验对烟气中的烟碱进行了定性分析。将该方法用于卷烟逐口抽吸时主流烟气的连续检测，结果显示，主流烟气的总离子(m/z 50~400)信号强度和烟碱离子(m/z 163)信号强度均随卷烟抽吸口数的增加而升高，这反映出卷烟主流烟气随烟支长度的释放规律。此外，对不同品牌卷烟的主流烟气进行 EESI-MS 在线检测，通过主成分分析(PCA)表明，该方法可以实现主流烟气的快速区分；通过聚类分析表明，该方法可以对主流烟气进行快速聚类和衡量其相似性。
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关键词:
- 电喷雾萃取电离源(EESI) /
- 卷烟主流烟气 /
- 烟碱 /
- 主成分分析(PCA) /
- 聚类分析
Abstract: Mainstream cigarette smoke (MCS)is very complex and highly dynamic aero-sol.Some substances in smoke are highly reactive,resulting in a continuously changing mixture during smoke processing of trapping and separation.Because the conventional off-line methods can alter the smoke composition,an online method of extractive elec-trospray ionization mass spectrometry (EESI-MS)was established for analyzing of MCS.The MCS,generated by a single channel smoking machine,was directly intro-duced into EESI-MS without sample preparation,and the mass spectrum was obtained. MS was operated at collision induced dissociation (CID)mode for qualitative analysis of nicotine.This method was applied for the MCS monitoring on a puff-by-puff basis,and the results showed that the signal intensity of total ions (m/z 50-400 )and ion of nicotine (m/z 1 63)increased with puffs,which indicated the trend of MCS emissions with the length of cigarette.Then,EESI-MS was used for analyzing of MCS of various cigarette brands.The result of principal component analysis (PCA)indicated that the EESI-MS method could be applied to differentiate MCS of various cigarette brands,the clustering analysis showed that the EESI-MS method could be applied to cluster the MCS of various cigarette brands and measure their similarity. -
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