Rapid quantification of 50 fatty acids in small amounts of biological samples for population molecular phenotyping

Figure 1. High-throughput quantitative fatty-acidomics analysis. A Transmethylation efficiency (12.5% CH₃COCl, 73 °C, 3 h) for different lipids including cholesteryl-esters (CE), triglycerides (TG), diglycerides (DG), lysophosphatidylcholines (LPC), phosphatidylcholines (PC), lysophosphatidylethanolamines (LPE), phosphatidylethanolamines (PE), phosphatidylglycerol (PG), free fatty acids (FFA), fatty acid esters of hydroxyl-fatty-acids (FAHFA), acylcarnitines (ACar), sphingomyelins (SM) and ceramides (Cer). B Isomerization of some representative unsaturated fatty acids as a function of temperature and CH₃COCl concentration. C concentrations (c, μmol/L) of fatty acids in 10 μL human fluids (urine and plasma) and 10 mg of other biological matrices (H1299 cells, mouse cecum content and rabbit liver tissue)

Figure 2. Total ion chromatogram (TIC) from GC-FID/MS analysis of 45 methylated FAs (Keys for analytes are listed in supplementary Table S3)

Figure 3. Correlations for experimental (t_R^E) and calculated values (t_R^C) from the quantitative structure-retention relationship (A) and prediction accuracy for six test analytes (B)