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Figure 1. Basic sample preparation workflow. It shows the main processes of sample preparation, starting from chemical fixation and ending with ultra-sectioning
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Figure 2. Differences of ultrastructure between the cryo-fixed and chemical-fixed synaptic samples. The observation of cryo-fixed and chemical-fixed neuropil from the cerebral cortex of an adult mouse indicates that there is a reduction of extracellular space (blue) after chemical fixation. Adapted from Korogod et al. (2015) with permission
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Figure 3. Cryo-ET imaging of neuronal α-Syn aggregates. A A tomographic slice shows the intracellular organization of a p62-RFP-expressing neuron, mitochondria (Mito), vesicles (Ves), endoplasmic reticulum (ER), autophagosome (Auto) and α-Syn fibers (labelled by red arrows) can be seen. Scale bar: 350 nm. Auto; autophagosome, ER; endoplasmic reticulum, Mito; mitochondrion, Ves; vesicles. B 3D rendering of the tomogram (panel A) shows the location and organization of α-Syn fibrils (red), an autophagosome (cyan), ER (yellow), mitochondria (green), and various vesicles (purple). Adapted from Trinkaus et al. (2021) with permission
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Figure 4. The soluble Tau can alter hippocampal granule neurons synapses and newborn granule neurons. Compared to the control group (A, C and E), the soluble Tau (B and D) has long-term influences on afferent synapses in the molecular layer (ML) with the lower density of synapses (red arrows), higher postsynaptic density (brackets). It also changes the morphology of newborn granule neurons (F). Red scale bar: 200 nm. White scale bar: 50 μm. ML: molecular layer; GL: granular layer; H: hilus. Adapted from Bolós et al. (2017) with permission
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Figure 5. Iron accumulation in R6/2 HD striatal neurons. There was abundant iron (II) staining in the cytoplasm of striatal neurons with the complicated membrane (A and B), while there were no differences in iron (III) in the striatum between WT and HD mice (C and D). Adapted from Chen et al. (2013) with permission
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