Figure 2 ,Table 6个

    • Figure 1.  Workflow of mouse cardiomyocyte isolation and patch clamp recording. A Langendorff perfusion system. Schematic representation of the Langendorff method for isolating cardiomyocytes. The heart is excised from the mouse, followed by perfusion with an enzyme-containing solution to dissociate cells. B Heart digestion. Image of the mouse heart being digested using the Langendorff perfusion system. The perfusion needle is inserted into the aorta, and the heart is perfused with collagenase solution. C Isolated cardiomyocytes. Light microscopy image showing isolated adult mouse cardiomyocytes. The cells are shown to disperse after enzymatic digestion. Scale bar, 50 μm. D Patch clamp setup. Image of a cardiomyocyte being patched with a glass micropipette. The cell is positioned under an inverted microscope, and the pipette is used to form a high-resistance seal for recording. Scale bar, 50 μm

    • Figure 2.  Patch clamp recording and data analysis of KATP channel currents. A Patch-clamp methodology. Schematic representation of the patch-clamp technique used to record KATP channel currents. The process begins with the placement of the recording pipette on a cardiomyocyte, followed by mild suction to form a giga-seal. Two configurations are shown: inside-out and whole-cell modes. The inside-out patch involves exposing the intracellular side of the channel to the extracellular environment, whereas the whole-cell patch configuration allows for the recording of currents from the entire cell. B Inside-out current recording. Representative trace of KATP channel currents recorded in the inside-out patch configuration in response to varying ATP concentrations. The ATP concentrations tested (in mmol/L) are indicated above the trace. C ATP sensitivity curve: Normalized current as a function of ATP concentration. The current is plotted on a logarithmic scale, demonstrating the inhibitory effect of ATP on KATP channel activity. D Single-channel current trace. High-resolution current trace showing unitary KATP channel openings and closings, recorded under conditions that allow single channel activity. The annotations on the trace indicate that "O" (Open) represents the channel's open state, and "C" (Close) represents the channel's closed state. E Current amplitude histogram. Distribution of single-channel current amplitudes, illustrating the presence of distinct conductance states. F Whole-cell current recording. Current trace showing KATP channel activity in whole-cell configuration. The trace includes the effects of pinacidil (Pin, a KATP channel opener) and glibenclamide (Glib, a KATP channel blocker). G Current–voltage relationship. Current–voltage (I–V) curve demonstrating the effect of pinacidil and glibenclamide on KATP channel currents. The I–V relationship is shown for control (NC), pinacidil-treated (Pin), and glibenclamide-treated (Glib) conditions