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重离子束12C6+累进辐照诱变柠檬酸菌株选育研究

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陈积红, 胡伟, 李文建, 刘敬, 王曙阳, 魏子昊. 2013: 重离子束12C6+累进辐照诱变柠檬酸菌株选育研究, 原子核物理评论, null(4): 483-487. doi: 10.11804/NuclPhysRev.30.04.483
引用本文: 陈积红, 胡伟, 李文建, 刘敬, 王曙阳, 魏子昊. 2013: 重离子束12C6+累进辐照诱变柠檬酸菌株选育研究, 原子核物理评论, null(4): 483-487. doi: 10.11804/NuclPhysRev.30.04.483
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CHEN Jihong, HU Wei, LI Wenjian, LIU Jing, WANG Shuyang, WEI Zihao. 2013: Breeding Study of Hyper Citric Acid Strain by Progressive Heavy Ion Irradiation, Nuclear Physics Review, null(4): 483-487. doi: 10.11804/NuclPhysRev.30.04.483
Citation: CHEN Jihong, HU Wei, LI Wenjian, LIU Jing, WANG Shuyang, WEI Zihao. 2013: Breeding Study of Hyper Citric Acid Strain by Progressive Heavy Ion Irradiation, Nuclear Physics Review, null(4): 483-487. doi: 10.11804/NuclPhysRev.30.04.483
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重离子束12C6+累进辐照诱变柠檬酸菌株选育研究

  • 中国科学院近代物理研究所，甘肃兰州 730000; 甘肃省微生物资源开发利用重点实验室，甘肃兰州 730000

Breeding Study of Hyper Citric Acid Strain by Progressive Heavy Ion Irradiation

  • 摘要: 应用大剂量重离子束12C6+对菌株H3001进行二次辐照选育，对初选获得的高产柠檬酸菌株进行摇瓶发酵试验及10～100 L中试发酵罐试验，采用酸碱中和法测定发酵液中柠檬酸的含量。结果表明：当二次重离子12C6+剂量为857.8 Gy时，致死率和正突变率达到最大值，分别为94.5%和8%。通过摇瓶发酵试验，最终获得一株高产柠檬酸菌株hw317，控制该菌株发酵周期为60 h，柠檬酸酸度能达到19.2±0.2%。
    Abstract: Heavy 12C6+ ion beams in various high doses were employed to irradiate H3001 strain for screening As-pergillus niger strain for hyper citric acid production. Three high-yield strains were obtained after shaker fermentation test. Among the three strains, the strain hw317 was implemented shaker fermentation for stability test and 10～100 L pilot fermentation tank for citric acid productive maximization. Acid-base neutralization method was applied to determi-nate the content of citric acid in fermented liquid. The results showed that: when the secondary heavy ion 12C6+ dose was 857.8 Gy, both of the fatality rate (94.5%) and the positive mutation rate (8%) were highest. Through the shaker fermentation tests and 10～100 L pilot fermentation test, one strain hw317 was screened and obtained for hyper citric acid production. Consequently, the final citric acid acidity can reach up to 19.2±0.2%with controlling fermentation cycle for 60 h.
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  • 刊出日期:  2013-12-20

重离子束12C6+累进辐照诱变柠檬酸菌株选育研究

  • 中国科学院近代物理研究所，甘肃兰州 730000; 甘肃省微生物资源开发利用重点实验室，甘肃兰州 730000

摘要: 应用大剂量重离子束12C6+对菌株H3001进行二次辐照选育，对初选获得的高产柠檬酸菌株进行摇瓶发酵试验及10～100 L中试发酵罐试验，采用酸碱中和法测定发酵液中柠檬酸的含量。结果表明：当二次重离子12C6+剂量为857.8 Gy时，致死率和正突变率达到最大值，分别为94.5%和8%。通过摇瓶发酵试验，最终获得一株高产柠檬酸菌株hw317，控制该菌株发酵周期为60 h，柠檬酸酸度能达到19.2±0.2%。

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