Keywords: 
• 杜仲 /
• 杜仲叶 /
• 杜仲雄花 /
• 液相色谱-串联三重四极杆飞行时间高分辨质谱(LC-TripleTOFMS/MS) /
• 化学成分

Abstract: In order to study the difference of synthesis and accumulation of metabolites in different medicinal parts of Eucommia ulmoides Oilv.(Eucommiae Cortex,Eucom-miae Folium and Eucommiae Flos Male),LC-Triple TOF MS/MS method combined with multivariate statistical analysis was used to analyze the difference of chemical con-stituents in different medicinal parts of Eucommia ulmoides Oilv.Through the analysis of the multistage tandem mass spectrometry,the characteristic peaks were extracted with mass spectrometry data peak matching,peak alignment,noise filtering.Hierachi-cal cluster analysis(HCA),principal component analysis(PCA)and partial least-squares discriminant analysis(PLS-DA)were used for data processing.The constitu-ents were identified according to MS accurate mass and MS/MS spectrometry fragmen-tation information,combined with the software of database search,comparison with reference standards and literature.The results showed that the chemical constituents in Eucommiae Cortex,Eucommiae Folium and Eucommiae Flos Male were clearly distin-guished.Kinds of 23 differential chemical constituents are screened and indentified, including aucubin,deacetyl asperulosidic acid,guaiacyl glycerol,geniposidic acid,chlo-rogenic acid, cryptochlorogenic acid, geniposide, caffeic acid, pinoresinol-di-O-β-D-glucopyranoside, rutin, kaempferol-3-O-rutinoside,isoquercitrin,eucomoside B, pinoresinol-4'-O-β-D-glucopyranoside,quercetin-3-O-malonyl-β-D-glucoside,astragalin, isochlorogenic acid A,kaempferol-3-O-(6″-O-acetyl)-β-D-glucopyranoside,naringenin, kaempherol and three kinds of quercetin glycoside,which present different changing laws.The different constituents among Eucommiae Cortex,Eucommiae Folium and Eucommiae Flos M ale can provide the material basis for revealing the property and effi-cacy of different medicinal parts of Eucommia ulmoides Oilv.