低能N离子注入对蚕豆根尖细胞的诱变效应
Effects of Irradiation with Low-energy Nitrogen Ion Injection on Root Tip Cells of Broad Bean
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摘要: 为了研究低能N离子束的细胞遗传学效应,以不同剂量的N离子束对蚕豆种子的种胚进行辐照,观察分析根尖细胞的微核率、有丝分裂指数和染色体畸变效应。研究发现,离子柬的注入抑制了根尖细胞的有丝分裂,干扰了正常的有丝分裂过程,引发了染色体的结构、行为和数目畸变;随着离子注入剂量的增加,微核率增加、有丝分裂指数降低、染色体畸变率增加。Abstract: In order to study the cytogenetic effects of low-energy nitrogen ion irradiation, broad bean seed embryo was irradiated by different doses of nitrogen ions. Micronucleus rate, mitotic index and chromo- some aberration in root-tip cells were analyzed. The results showed that the injection of ions inhibited mi- tosis of root tip cells, interfered the normal process of mitosis, caused aberrations of chromosome struc- ture, behavior and number. The frequency of micronucleus and chromosomal aberrations increased with the increasing radiation dosage, while mitotic index decreased.
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