高级搜索

阿佛曼链霉菌的~(12)C离子辐照效应及高产菌株选育

,  ,  ,  ,  ,  ,  , 

downloadPDF

上一篇

下一篇

王曙阳, 陈积红, 李文建, 刘敬, 曹云飞, 薄永恒, 马晓琪, 梁剑平. 2010: 阿佛曼链霉菌的~(12)C离子辐照效应及高产菌株选育, 原子核物理评论, 27(1): 82-86.
引用本文: 王曙阳, 陈积红, 李文建, 刘敬, 曹云飞, 薄永恒, 马晓琪, 梁剑平. 2010: 阿佛曼链霉菌的~(12)C离子辐照效应及高产菌株选育, 原子核物理评论, 27(1): 82-86.
shu
WANG Shu-yang, CHE Ji-hong, LI Wen-jian, LIU Jing, CAO Yun-fei, BO Yong-heng, MA Xiao-qi, LIANG Jian-ping. 2010: Research on Mutation Breeding of High-producing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of ~(12)C~(6+), Nuclear Physics Review, 27(1): 82-86.
Citation: WANG Shu-yang, CHE Ji-hong, LI Wen-jian, LIU Jing, CAO Yun-fei, BO Yong-heng, MA Xiao-qi, LIANG Jian-ping. 2010: Research on Mutation Breeding of High-producing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of ~(12)C~(6+), Nuclear Physics Review, 27(1): 82-86.
shu

阿佛曼链霉菌的~(12)C离子辐照效应及高产菌株选育

  • 中国科学院近代物理研究所,甘肃,兰州,730000

  • 甘肃农业大学,甘肃,兰州,730070

Research on Mutation Breeding of High-producing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of ~(12)C~(6+)

  • 摘要: 选用~(12)C~(6+)离子辐照诱变阿维菌素B1a产生菌ZJAV-A1,研究其诱变效应.实验结果表明,~(12)C~(6+)离子辐照剂量50 Gy时致死率97%,正突变率最高可达到34.2%.通过~(12)C~(6+)离子诱变处理,结合平板培养基及斜面培养基的正突变菌株筛选,最终获得一株稳定性良好,阿维菌素B1a组分产量稳定在4460-4588μg/ml之间,较出发菌株提高11.1%-14.7%的突变株ZJAV-Y1-203.
    Abstract: Mutagenic effect on avermectin Bla producing strains of ZJAV-A1 by ion beam of ~(12)C~(6+) has been investigated.The experimental results indicated that the lethality was 97% and the highest rate of orthomutation was 34.2%,when ZJAV-A1 was irradiated by ion beam of 50 Gy ~(12)C~(6+).After the mutagenesis processing by ion beam of ~(12)C~(6+) and the screening of orthomutation strains by using plating mediums and slant cultures,the mutant ZJAV-Y1-203 was obtained with the avermectin Bla yield of 4588-4460 μg/ml.Compared with the original strain,the titer was improved 11.1%-14.7%.
  • 加载中
  • 加载中
计量
  • 文章访问数:  338
  • HTML全文浏览数:  40
  • PDF下载数:  0
  • 施引文献:  0
出版历程
  • 刊出日期:  2010-03-20

阿佛曼链霉菌的~(12)C离子辐照效应及高产菌株选育

  • 中国科学院近代物理研究所,甘肃,兰州,730000
  • 甘肃农业大学,甘肃,兰州,730070

摘要: 选用~(12)C~(6+)离子辐照诱变阿维菌素B1a产生菌ZJAV-A1,研究其诱变效应.实验结果表明,~(12)C~(6+)离子辐照剂量50 Gy时致死率97%,正突变率最高可达到34.2%.通过~(12)C~(6+)离子诱变处理,结合平板培养基及斜面培养基的正突变菌株筛选,最终获得一株稳定性良好,阿维菌素B1a组分产量稳定在4460-4588μg/ml之间,较出发菌株提高11.1%-14.7%的突变株ZJAV-Y1-203.

English Abstract

    全文HTML

参考文献 (0)

目录

/

返回文章
返回